Part:BBa_K4358001

Xylose repressor

xylose represser

Gene expression with xylose promoter can be induced by the addition of xylose. When xylose is present, xylose will act as an inducer and bind to the xylose regulator protein to change its structure. Hence, xylR won’t bind to the operator(XylR-binding site) to inhibit the downstream gene of the xylR-binding site.

So we designed the plasmid with Pxyl, XylR and XylR-binding site. Then we transformed pqqC, pqqD and pqqE in the downstream of the xylR-binding site in addition to control the expression of pqqC, pqqD and pqqE to let more resources be used for the biosynthesis of PqqA and PqqB.

Figure1. It illustrated the mechanism of how xylose regulator protein interacts with xylose promoter and inhibits downstream gene’s performance. The XylR binds to the xylose binding site until the structure changes because of xylose.

Figure 2. The 3D structure of xylose repressor

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 870
  Illegal XbaI site found at 121
  Illegal XbaI site found at 961
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 870
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 870
  Illegal XhoI site found at 838
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 870
  Illegal XbaI site found at 121
  Illegal XbaI site found at 961
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 870
  Illegal XbaI site found at 121
  Illegal XbaI site found at 961
  
• 1000
  COMPATIBLE WITH RFC[1000]